How Is PCR Used In Different Industries
PCR (Polymerase Chain Reaction) is a technique which is used extensively in life sciences laboratories along with drug research and development processes in the pharmaceutical industry. The process, especially the variation of hot start PCR is rapidly coming into common utilization in these industries and possesses a variety of applications in medicine and genetic research. Two of the most common applications for PCR are given below, along with brief explanations.
Diagnosis
This is a growing division of use for the technique and something which holds a great deal of promise for the diagnosis, management and treatment of|management of|treatments for} a number of diseases including lymphomas and leukemia. The malignant cells which characterize these diseases might be identified through PCR assays, while using telltale signs and symptoms of malignancy being identifiable through the existence ofthe use of translocation-specific cells.
PCR can be useful for the diagnosis of fungal and viral infections, so long as the primers utilized in the assay are specifically geared to act with the viral, fungal, or other pathogenic DNA. The high sensitivity of this method enables earlier detection thereby, earlier and much more aggressive treatment of serious illnesses.
Selective Isolation of DNA
By selectively amplifying targeted DNA sequences from genomic DNA, PCR can isolate selected sequences, an application which can be used in DNA cloning (which PCR’s generally high yield is very perfect for) along with forensic science procedures.
Of course, the aforementioned Hot start PCR is a process with similar implications, albeit a modified version. This method can result in greater product yields through turning the taq polymerase inactive by lowering the temperature – then increasing it again with an additional primer to commence the amplification process once more.
Small examples of DNA may be amplified from the PCR method to generate ample DNA material to serve for the purpose of genetic fingerprinting, which is probably the most accurate means of identification.
The identical technique is also a good choice for paternity testing and also research into the genetic relationships between different species, providing continual understanding of the entire process of evolution along with the science of genetics all together.
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